Nikon A1 Confocal Laser Microscope System + PicoQuant

Category: Equipment

Nikon A1 Confocal Laser Microscope System

Nikon A1 Confocal Laser Microscope System

The Nikon A1 is a basic confocal system consisting of a separate scan head, standard 5 PMT detectors, control module, and laser module. The A1 has a standard high-resolution confocal mode up to 4096 X 4096 pixels. This laser scanning confocal system is similar to that of the Nikon A1R in that it is configured on the Nikon TiE Widefield, inverted, fluorescence microscope. NIS Elements imaging software is used to control acquisition and analysis.

Key features include:

  • 4 laser lines
    • 405 nm diode at 38mW
    • 488 nm diode at 20 mW
    • 561 nm diode at 40mW
    • 638 nm diode at 10mW
  • 5 PMTs (photomultiplier tubes)
  • Autofocus system
  • Nikon TiE Wide field fluorescence microscope
  • Objectives
    • 10X Plan-Fluor/0.3 NA w/ phase
    • 20X Plan-Apo/0.75 NA

      K. Venkatachalam. Drosophila Larval brain stained for nociceptive neurons (green) and serotonin (red)

    • 40X Plan-Fluor/0.75 NA
    • 40X Plan-Fluor/1.3 NA Oil
    • 60X PApo TIRF/1.49 NA Oil
    • 100X Plan-Apo/1.4 NA Oil

The PicoQuant Imaging System is an upgrade package added to the Nikon A1 system for the purpose of performing Fluorescence Lifetime Imaging (FLIM). This system employs pulsed lasers for the purpose of assessing protein-protein interactions and is unique to the IBP Imaging Core. The system is equipped with 439 nm and 483 nm  lasers.

This equipment is located in MSB 4.532.

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