Assistant Professor Pediatrics, Pediatric Research Center, Medical School
Education & Training
- Postdoctoral Fellow
- M.D. Anderson Cancer Center, The University of Texas Health Science Center, Houston, 2012
- Genetics & Molecular Biology, Emory University, Atlanta, GA, 2007
Areas of Interests
- Research Interest
- Kidney development, Planar cell polarity and Wnt signaling, Primary cilia, Morphogenesis, Frog Embryogenesis
Wnt/planar cell polarity signaling in kidney development
Our studies focus on how Wnt signaling modulates cell shape and organization to facilitate the formation of tubules. We utilize frog (Xenopus) embryonic kidneys and mammalian cultured cells as models. Cells perceive their orientation within a tissue through a specific Wnt signaling pathway called planar cell polarity (PCP). Our studies focus on how PCP components with cytoskeletal roles regulate tubulogenesis.
Cystic kidneys are the most common feature of a group of genetic diseases known as ciliopathies. They result from defects in the primary cilia projecting into nephron lumens. Primary cilia act as cellular antennae, orchestrating cellular signaling and orienting cells through PCP. We are interested in the role of PCP and primary cilia in shaping nephric tubules.
- DeLay BD, Krneta-Stankic V, Miller RK. Technique to target microinjection to the developing Xenopus kidney. JoVE. In Press.
- Krneta-Stankic V, DeLay B, Miller RK. Xenopus: Leaping forward in kidney organogenesis. Pediatric Nephrology. In Press.
- Miller, Rachel K., McCrea, P.D. “The Xenopus Pronephros: A Kidney Model Making Leaps toward Understanding Tubule Development.” Xenopus Development. By M. Kloc. 1st ed. Oxford: Wiley & Sons, 2014. (pp.215-38). Print.
- Chang, D. R., Martinez Alanis, D., Miller, R. K., Ji, H., Akiyama, H., McCrea, P. D., & Chen, J. (2013). Lung epithelial branching program antagonizes alveolar differentiation. Proceedings of the National Academy of Sciences of the United States of America, 110(45), 18042–18051. doi:10.1073/pnas.1311760110.
- Miller, R. K., Hong, J. Y., Muñoz, W. A., & McCrea, P. D. (2013). Beta-catenin versus the other catenins: assessing our current view of canonical Wnt signaling. Progress in Molecular Biology and Translational Science, 116, 387–407. doi:10.1016/B978-0-12-394311-8.00017-0.
- Miller, R. K., Gomez de la Torre Canny, S., Jang, C.-W., Cho, K., Ji, H., Wagner, D. S., … McCrea, P. D. (2011). Pronephric Tubulogenesis Requires Daam1-Mediated Planar Cell Polarity Signaling. Journal of the American Society of Nephrology : JASN, 22(9), 1654–1664. doi:10.1681/ASN.2010101086.
- Miller, R. K., & McCrea, P. D. (2010). Wnt to Build a Tube: Contributions of Wnt signaling to epithelial tubulogenesis. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 239(1), 77–93. doi:10.1002/dvdy.22059.
- Lyons, J. P., Miller, R. K., Zhou, X., Weidinger, G., Deroo, T., Denayer, T., … McCrea, P. D. (2009). Requirement of Wnt/beta-catenin signaling in pronephric kidney development.. Mechanisms of Development, 126(3-4), 142–159. doi:10.1016/j.mod.2008.11.007.