Areas of Interests
- Research Interest
- Lab Research
The kidney is the primary organ for maintaining K+ homeostasis of the body. This is accomplished by closely regulating K+ excretion to match K+ intake under normal physiological states. Renal control of K+ secretion occurs predominantly in the late distal tubule, notably the connecting tubule (CNT) and cortical collecting duct (CCD), where K+ secretion is tightly controlled. This is thought to be mediated by two types of K+ channels: the renal outer medullary K+ channel (ROMK, Kir1.1), and the large conductance, voltage- and Ca2+-activated K+ channel (BK, maxi-K+ channel). It is general considered that the ROMK channel plays a dominant role in maintaining basal levels of K+ secretion. In contrast, the BK channel activity is typically low under basal conditions, but is rapidly stimulated during certain stressed states. Our research have shown that elevated shear stress activate the mechanosensitive TRPV4 channel in these segments, leading to rapid influx of Ca2+ with subsequent activation of BK to give rise to flow-dependent K+ secretion. Current study is to investigate other Ca2+-activated K+ channels expression in the CNT and CCD, and to determine the roles in Ca2+-dependent secretion in the kidney.
My other research interest is the consequences of protein modifications (SUMO) induced cell signaling and cellular functions change with special focus of cancer development. Covalent modification of small ubiquitin (Ub)-like modifier (SUMO) proteins were first discovered in 1996. SUMOs are 11 kDa proteins that only share 18% sequence identity with ubiquitin, but are very similar to the ubiquitin folded structure. SUMOs are highly conserved protein family in all eukaryotes, including yeast, and are essential for almost all of them. SUMO modifications (SUMOylation) have been found in many proteins, including the androgen receptor, IκBα, c-jun, histone deacetylases (HDACs) and p53. SUMOs play roles in all tissues and at all developmental stages; contribute to such diverse processes as transcription, DNA repair, nuclear transport and cell cycle progression. SUMO have been demonstrated to play important roles in cancer development, including prostate cancer, thyroid cancer, colon cancer, pancreatic cancer and breast cancer. Our current study is to understand SUMO functions and to identify new potential therapeutic target in prostate cancer.
- Yue Li, Hongxiang Hu, Michael B. Butterworth, Jin-Bin Tian, Michael X. Zhu1, and Roger G. O’Neil. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney, PLOS ONE, In press.
- Feng-Ming Lin, Santosh Kumar, Jing Ren, Samaneh Karami, Shaymaa Bahnassy, Yue Li, Xiaofeng Zheng, Jing Wang and Tasneem Bawa-Khalfe. SUMOylation of HP1α supports association with ncRNA to define responsiveness of breast cancer cells to chemotherapy. Oncotarget. 2016 Apr 14.
- Yue Li, Ikjin Kim, Chang Liu, Hai Rao. Rad4 Regulates Protein Turnover at a Postubiquitylation Step, Molecular Biology of the Cell, 2010 Jan; 21(1):177-85.
- Ikjin Kim, Yue Li, Paulina Muniz, Hai Rao. Usa1 Protein Promotes Substrate Ubiquitylation Through Two Separate Domains, PLOS ONE 29 Oct 2009 | doi:10.1371/journal.pone.0007604.
- Chang Liu*, Dewald van Dyk*, Yue Li, Brenda Andrews, Hai Rao. A synthetic dosage lethality screen reveals multiple pathways that require the functioning of ubiquitin-binding proteins Rad23 and Dsk2. BMC Biology, 2009 Nov 12;7:75.
- Yue Li, Yu Sun, Jane C. Hines, and Dan S. Ray. Identification of New Kinetoplast DNA Replication Proteins in Trypanosomatids Based on Predicted S-Phase Expression and Mitochondrial Targeting, Eukaryot Cell. 2007 Dec; 6(12): 2303-10.
- Yue Li, Zhao-Zhao Jiang, Wai-Keung Leung, Joseph JY Sung, Wei-Jun Ma. The Existence of a Putative Regulatory Element in 3’-Untranslated Region of Proto-oncogene HOX11’s mRNA, Journal of Biochemistry and Molecular Biology, 2005, 38(4): 500-506.
- Yue Li, Zhaozhao Jiang, Haixu Chen, Wei-Jun Ma. A Modified Quantitative EMSA and its Application in the Study of RNA-protein Interactions, Journal of Biochemical and Biophysical Methods, 2004, 60: 85-96.
- Li Yue, Chen Ceshi, Yin Guanglin. Studies on Purification and Characterization of 2,5-DKG Reductase I from ER97, Microbiology China, 2005, 32(6): 63-67.
- Li Yue, Chen Ceshi, Yin Guanglin. Cloning and Expression of 2,5-diketo-D-gluconate (2,5-DKG) Reductase Gene in Escherichia Coli, Microbiology China, 1999, 26:260-265.
- Hai-xu Chen, Yue Li, Zhao-zhao Jiang, Xian-ming Qu, Sheng-li Yang, Wei-jun Ma. The existence of putative post-transcriptional regulatory element in 3’-UTR of drosophila antibacterial peptide diptericin’s mRNA. Febs lett, 2004, 561: 181-185.
- Li Yue, Yan Ming, Yin Guanglin. Application of Computerized Gel Imaging System in Research of Bioengineering, Chinese Journal of Modern Scientific Instruments, 2000, 6: 29-30.
- Yan Ming, Li Yue, Yin Guanglin. Application of Computer Science in Molecular Biology, Chinese Journal of Modern Scientific Instruments, 2000, 4: 15-17.
- Chen Fang, Chen Ceshi, Li Yue, Yin Guanglin. Studies on Gene Knocking out of 2-keto aldose Reductases from Erwinia SP. SCB125, Acta Microbiologica Sinica, 2000, 40:475-481.
- Qiao Chunhong, Chen Ceshi, Chen Fang, Li Yue, Yin Guanglin, Production of 2-KLG from glucose by constructing recombinant bacteria, Industry Microbiology, 2000, 30 (1): 9-14.