Nikon AX-R Confocal Laser Microscope System @ IMM

The new generation AXR is a modular confocal system containing Ti2-E microscope, four laser unit, scan head, and detector module with two tunable detectors for green and red and two detectors with filters for blue and far red. The system has a spectral detector module and additional PMT for pseudo-DIC imaging. Control module manages the microscope, motorized stage and peripherals.

This Nikon platform also utilizes a high-end digital Color camera Fi3 for non-confocal image acquisition.AXR has two confocal modes: Resonant (high speed) and standard Galvano (slower, capable of scanning 8192 x 8192 pixels). The Resonant scanning minimizes disturbance to samples, and has large field of view 25 mm and band of scanning options with resolution up to 2048 x 2048 pixels. Setup of illumination, detectors, and bandpasses for both modes is done logically and automatically.

Key features include:

• 405 nm LD 15mW
• 488 nm LD 15mW
• 561 nm LD 15mW
• 640 nm LD 15mW

• 2X PLAN APO/0.10 NA
• 4X PLAN APO/0.20 NA
• 10X PLAN APO/0.45
• 20X PLAN APO/0.80 NA
• 40X APO LWD/1.15 NA Water
• 60X PLAN APO/ 1.42 NA Oil