Nikon A1 Confocal Laser Microscope System + PicoQuant

The Nikon A1 is a basic confocal system consisting of a separate scan head, standard 5 PMT detectors, control module, and laser module. The A1 has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise, and allow less phototoxicity.  High-resolution confocal mode permits up to 4096 X 4096 pixels. This laser scanning confocal system is similar to that of the Nikon A1R in that it is configured on the Nikon TiE Widefield, inverted, fluorescence microscope. NIS Elements imaging software is used to control acquisition and analysis.

The PicoQuant Imaging System is an upgrade package added to the Nikon A1 system for the purpose of performing Fluorescence Lifetime Imaging (FLIM). This system employs pulsed lasers for the purpose of assessing protein-protein interactions and is unique to the IBP Imaging Core. The system is equipped with 439 nm (121 mW peak power) and 483 nm (136 Mw peak power) lasers.

  • 5 PMTs (photomultiplier tubes)
  • Autofocus system
  • Nikon TiE Wide field fluorescence microscope

Key features include:

4 laser lines Objectives
  • 405 nm diode at 100 mW
  • 488 nm diode at 350 mW
  • 561 nm diode at 480 mW
  • 640 nm diode at 100 mW
  • 10X Plan-Fluor/0.3 NA w/ phase
  • 20X Plan-Apo/0.75 NA
  • 40X Plan-Fluor/0.75 NA
  • 40X Plan-Fluor/1.3 NA Oil
  • 60X PApo TIRF/1.49 NA Oil
  • 100X Plan-Apo/1.4 NA Oil