Lambert LIFA Fluorescence Lifetime Imaging Microscopy System

The Lambert FLIM system is a fully developed system for frequency domain measurement of fluorescence lifetime with high accuracy, stability and acquisition/processing speed.

 

Key features include:

Nikon TiE Widefield fluorescence microscope
High power multi-LED unit with peak emissions at: Objectives
  • 405nm (BFP)
  • 447nm (CFP)
  • 479nm (GFP)
  • 532nm (RFP)
  • 10X Plan-Fluor/0.45 NA w/phase
  • 20X Plan-Apo/0.75 NA
  • 40X Plan-Fluor/1.30 NA Oil
  • 60X Plan-Apo/1.40 NA Oil

 

Calcium Rig

Picture of Calcium Rig
The Nikon TiE Imaging System is a high resolution system designed for fluorescence imaging of small molecules in living cells. It is a filter-based, wide-field fluorescence system designed for measurement of intracellular signaling molecules including calcium ions, hydrogen ions (pH), nitric oxide, and other small molecules using appropriate fluorophores. Nikon’s NIS Elements imaging software is used to control acquisition and analysis of captured images. The software features include capabilities for single wavelength, multiple wavelength, and ratiometric mode fluorescence imaging.

 

Key features include:

6 laser lines Objectives
  • 405 nm LD 20 mW
  • 445 nm LD 20 mW
  • 488 nm DPSS 70mW
  • 514 nm DPSS 40 mW
  • 561 nm DPSS 70mW
  • 647 nm LD 125 mW
  • CFI PLAN APO LAMBDA 4X
  • CFI PLAN APO LAMBDA 10X
  • CFI PLAN APO LAMBDA 20X
  • CFI PLAN FLUOR 40X OIL NA1.30 WD 0.20MM
  • CFI HP APO TIRF AC 100X OIL