Equipment

Nikon N-STORM Super-Resolution Microscope System with TIRF

This new Nikon n-STORM Super-Resolution system is tailored toward single molecule imaging using Stochastic Optical Reconstruction Microscopy (STORM) and/or PhotoActivated Localization Microscopy (PALM) with high accuracy localization to create images with spatial resolution up to 20nm (10-fold over conventional optical microscopy) and is capable of multi-color imaging in both 2D and 3D modes. Total Internal Reflection Fluorescence (TIRF) microscopy capabilities.
Key features include:
6 laser lines	Objectives
405 nm LD 20 mW 445 nm LD 20 mW 488 nm DPSS 70mW 514 nm DPSS 40mW 561 nm DPSS 70mW 647 nm LD 125 mW	CFI PLAN APO LAMBDA 4X CFI PLAN APO LAMBDA 10X CFI PLAN APO LAMBDA 20X CFI PLAN FLUOR 40X OIL CFI HP APO TIRF AC 100X OIL

Nikon N-SIM Structured Illumination Super-Resolution Microscope System

The system uses Structured Illumination technology, which gives a two-fold enhancement in resolution (over conventional optical microscopy). Has ability to image dynamic events in live samples in both 2D, 3D and TIRF-SIM (higher signal-to-noise to enhance resolution even further). Includes top of the line super-resolution and TIRF objectives (100X) have superior optics with the least amount of lens defects/aberrations. A wide-ranging illumination and detection systems to give users greater flexibility.
Key features include:
6 laser lines	Objectives
405 nm LD 20 mW 445 nm LD 20 mW 488 nm DPSS 70mW 514 nm DPSS 20 mW 561 nm DPSS 70mW 647 nm LD 125 mW	CFI PLAN APO LAMBDA 10X CFI PLAN APO LAMBDA 20X CFI HP APO TIRF AC 100X OIL

Nikon A1R Confocal Laser Microscope System

The Nikon A1R is a modular confocal system consisting of a separate scan head, standard 4 PMT detector module plus an additional PMT for pseudo-DIC imaging, control module, laser module, and spectral detector module. The A1R has a standard high-resolution confocal mode up to 4096 x 4096 pixels as well as a high-speed resonant mode capable of scanning 230 frames per sec at 512 x 64 pixels. In addition to laser-based, confocal image acquisition, this Nikon platform also utilizes a high-end digital camera for non-confocal image acquisition. The updated A1R has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise ratio, and less phototoxicity. The updated A1R has the ability to perform Enhanced Resolution algorithms on point scanning confocal datasets to further increase resolution of images, and also Retirement/Replacement of Confocal Workstations allows for future expansion and faster processing, including GPU 3D Rendering.
Key features include:
6 laser lines	Objectives
405 nm LD 20 mW 445 nm LD 20 mW 488 nm DPSS 70mW 514 nm DPSS 40 mW 561 nm DPSS 70mW 640 nm LD 125 mW	10X Plan-Fluor/0.3 NA w/Phase 20X Plan-Apo/0.75 NA 40X Plan-Fluor/0.75 NA 40X Plan-Fluor/1.3 NA Oil 60X Apo TIRF/1.49 NA Oil 100X Plan-Apo/1.4 NA Oil

Nikon AX-R Confocal Laser Microscope System @ IMM

Located in the Center for Advanced Microscopy Annex at the Institute for Molecular Medicine The new generation AXR is a modular confocal system containing Ti2-E microscope, four laser unit, scan head, and detector module with two tunable detectors for green and red and two detectors with filters for blue and far red. The system has a spectral detector module and additional PMT for pseudo-DIC imaging. Control module manages the microscope, motorized stage and peripherals. This Nikon platform also utilizes a high-end digital Color camera Fi3 for non-confocal image acquisition.AXR has two confocal modes: Resonant (high speed) and standard Galvano (slower, capable of scanning 8192 x 8192 pixels). The Resonant scanning minimizes disturbance to samples, and has large field of view 25 mm and band of scanning options with resolution up to 2048 x 2048 pixels. Setup of illumination, detectors, and bandpasses for both modes is done logically and automatically.
Key features include:
4 laser lines	Objectives
405 nm LD 15mW 488 nm LD 15mW 561 nm LD 15mW 640 nm LD 15mW	2X PLAN APO/0.10 NA 4X PLAN APO/0.20 NA 10X PLAN APO/0.45 20X PLAN APO/0.80 NA 40X APO LWD/1.15 NA Water 60X PLAN APO/ 1.42 NA Oil

Nikon A1 Confocal Laser Microscope System + PicoQuant

The Nikon A1 is a basic confocal system consisting of a separate scan head, standard 5 PMT detectors, control module, and laser module. The A1 has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise, and allow less phototoxicity. High-resolution confocal mode permits up to 4096 X 4096 pixels. This laser scanning confocal system is similar to that of the Nikon A1R in that it is configured on the Nikon TiE Widefield, inverted, fluorescence microscope. NIS Elements imaging software is used to control acquisition and analysis. The PicoQuant Imaging System is an upgrade package added to the Nikon A1 system for the purpose of performing Fluorescence Lifetime Imaging (FLIM). This system employs pulsed lasers for the purpose of assessing protein-protein interactions and is unique to the IBP Imaging Core. The system is equipped with 439 nm (121 mW peak power) and 483 nm (136 Mw peak power) lasers. 5 PMTs (photomultiplier tubes) Autofocus system Nikon TiE Wide field fluorescence microscope
Key features include:
4 laser lines	Objectives
405 nm diode at 100 mW 488 nm diode at 350 mW 561 nm diode at 480 mW 640 nm diode at 100 mW	10X Plan-Fluor/0.3 NA w/ phase 20X Plan-Apo/0.75 NA 40X Plan-Fluor/0.75 NA 40X Plan-Fluor/1.3 NA Oil 60X PApo TIRF/1.49 NA Oil 100X Plan-Apo/1.4 NA Oil

Lambert LIFA Fluorescence Lifetime Imaging Microscopy System

The Lambert FLIM system is a fully developed system for frequency domain measurement of fluorescence lifetime with high accuracy, stability and acquisition/processing speed.
Key features include:
Nikon TiE Widefield fluorescence microscope
High power multi-LED unit with peak emissions at:	Objectives
405nm (BFP) 447nm (CFP) 479nm (GFP) 532nm (RFP)	10X Plan-Fluor/0.45 NA w/phase 20X Plan-Apo/0.75 NA 40X Plan-Fluor/1.30 NA Oil 60X Plan-Apo/1.40 NA Oil

Calcium Rig

The Nikon TiE Imaging System is a high resolution system designed for fluorescence imaging of small molecules in living cells. It is a filter-based, wide-field fluorescence system designed for measurement of intracellular signaling molecules including calcium ions, hydrogen ions (pH), nitric oxide, and other small molecules using appropriate fluorophores. Nikon’s NIS Elements imaging software is used to control acquisition and analysis of captured images. The software features include capabilities for single wavelength, multiple wavelength, and ratiometric mode fluorescence imaging.
Key features include:
6 laser lines	Objectives
405 nm LD 20 mW 445 nm LD 20 mW 488 nm DPSS 70mW 514 nm DPSS 40 mW 561 nm DPSS 70mW 647 nm LD 125 mW	CFI PLAN APO LAMBDA 4X CFI PLAN APO LAMBDA 10X CFI PLAN APO LAMBDA 20X CFI PLAN FLUOR 40X OIL NA1.30 WD 0.20MM CFI HP APO TIRF AC 100X OIL

IVIS LUMINA

The IVIS Lumina XR is live, whole animal imaging system capable of performing longitudinal fluorescence, bioluminescence and X-ray scans on in vivo and ex vivo subjects. The IVIS Lumina XR is capable of imaging all common fluorescent and bioluminescent reporters/dyes ranging from green (~488 nm) to near-infrared. The unit also contains an isoflurane-based, gas anesthesia system used for live animal studies, though investigators may utilize other methods of anesthesia provided they are approved and documented by the UT-IACUC.
Key features include:
IVIS Lumina XR	X-Ray Module
High-sensitivity in vivo and in vitro imaging of fluorescence and bioluminescence Back-thinned, back-illuminated grade 1 CCD provides high quantum efficiency over the entire visible to near-infrared spectrum Light-tight imaging chamber 8 position emission filter wheels 10 position excitation filter wheels 5 filter wheel choices for a broad range of fluorescence applications LED lamps for photographic images Heated stage to maintain optimum body temperature Automated motor controlled stage, filter wheel, lens position, and f-stop	Average X-ray acquisition in less than 10 seconds Radiation shielded Cabinet Exceeds standards set by the US FDA Center for Devices and Radiological Health (21 CFR-1020.40) Automated image integration to overlay with Bioluminescence, Fluorescence and Photograph
optical zoom lens attachment for close up and high-resolution X-ray

Data Processing Computers:

Workstation 1: Nikon Advanced Super-Resolution Image Analysis

Super Resolution Workstation

Workstation 2: General Image Analysis:

picture of Data processing computer

NIS Elements AR; Meta Morph; LIFA-FLIM; Living Image; Image J; Slidebook; AutoQuant, and Adobe Photoshop elements 2.0.