Publications

Ge, X., and Li, Q.: Humoral Immune Responses to Human Cardiac Troponin T by Genetic Immunization. Chin. J. Microbiol. Immunol. 23[7]:544-547, 2003.

Ge X., Yang, D.S.C., Trabbic-Carlson, K., Kim, B., Chilkoti, A., and Filipe, C.D.M.: Self-Cleavable Stimulus Responsive Tags for Protein Purification Without Chromatography. J. Am. Chem. Soc. 127[32]:11228-11229, 2005.

  • Chemical & Engineering News 83[32]:36, 2005
  • The Scientist 19[22]:24, 2005
  • Faculty of 1000 Biology 16089436

Ge, X., Trabbic-Carlson, K., Chilkoti, A., and Filipe C.D.M.: Purification of an Elastin Like Fusion Protein by Microfiltration. Biotechnol. Bioeng. 95[3]:424-432, 2006.

Ge, X., and Filipe, C.D.M.: Simultaneous Phase Transition of ELP Tagged Molecules and Free ELP: An Efficient and Reversible Capture System. Biomacromolecules. 7[9]:2475-2478, 2006.

Ge, X., Conley, A., Brandle, J.E., Truant, R., and Filipe C.D.M.: In Vivo Formation of Protein Based Aqueous Microcompartments. J. Am. Chem. Soc. 131[25]:9094-9099, 2009.

Ge, X., Hoare, T., and Filipe, C.D.M.: Protein Based Aqueous-Multiphasic Systems. Langmuir. 26[6]:4087-4094, 2010.

Ge, X., Mazor, Y., Hunicke-Smith, S.P., Ellington, A.D., Georgiou, G.: Rapid Construction and Characterization of Synthetic Antibody Libraries Without DNA Amplification. Biotechnol. Bioeng. 106[3]:347-357, 2010.

Reddy, S.T., Ge, X., Miklos, A.E., Hughes, R.A., Kang, S.H., Hoi, K.H., Chrysostomou, C., Hunicke-Smith, S.P., Iverson, B.L., Tucker, P.W., Ellington, A.D., and Georgiou, G.: Monoclonal Antibodies Isolated Without Screening by Analyzing the Variable-Gene Repertoire of Plasma Cells. Nat. Biotechnol. 28[9]:965-969, 2010.

Cell 143:491, 2010

Biotechniques News 10/5/2010

Ge, X., Lebert, J.M., Monton, M.N., Lautens, L.L., and Brennan, J.D.: Materials Screening for Sol-Gel Derived High-Density Multi-Kinase Microarrays. Chem. Mater. 23[16]:3685-3691, 2011.

Ippolito, G.C., Hoi, K.H., Reddy, S.T., Carroll, S.M., Ge, X., Rogosch, T., Zemlin, M., Shultz, L.D., Ellington, A.D., Vandenberg, C.L., and Georgiou, G.: Antibody Repertoires in Humanized NOD-scid-IL2Rγ(null) Mice and Human B Cells Reveals Human-Like Diversification and Tolerance Checkpoints in the Mouse. PLoS One. 7[4]:e35497, 2012.

Eleftheriou, N.M., Ge, X., Kolesnik, J., Falconer, S.B., Harris, R.J., Khursigara, C., Brown, E.D., and Brennan J.D.: Entrapment of Living Bacterial Cells in Low-Concentration Silica Materials Preserves Cell Division and Promoter Regulation. Chem. Mater. 25[23]:4798-4805, 2013.

Ge, X., Eleftheriou, N.M., Dahoumane, S.A., and Brennan, J.D.: Sol-Gel Derived Materials for Production of Pin-Printed Reporter Gene Living-Cell Microarrays. Anal. Chem. 85[24]:12108-12117, 2013.

Nam, D.H., and Ge. X.: Development of Periplasmic FRET-Based Screening Method for Protease Inhibitory Antibodies. Biotechnol. Bioeng. 110[11]:2856-2864, 2013.

Haage, A., Nam, D.H., Ge, X., and Schneider, I.C.: Matrix Metalloproteinase-14 is a Mechanically Regulated Activator of Secreted MMPs and Invasion. Biochem. Biophys. Res. Commun. 450[1]:213-218, 2014.

Nam, D.H., and Ge, X.: Direct Production of Functional Matrix Metalloproteinase-14 Without Refolding or Activation and its Application for In Vitro Inhibition Assays. Biotechnol. Bioeng. 113[4]:717-723, 2016.

Li, X.C., Wang, C., Mulchandani, A., and Ge, X.: Engineering Soluble Human Paraoxonase 2 for Quorum Quenching. ACS Chem. Biol. 11[11]:3122-3131, 2016.

Nam, D.H., Rodriguez, C., Remacle, A.G., Strongin, A.Y., and Ge, X.: Active-Site MMP-Selective Antibody Inhibitors Discovered from Convex Paratope Synthetic Libraries. Proc Natl Acad Sci U. S. A. 113[52]:14970-14975, 2016.

  • Reported by AAAS Eurekalert, Genetic Engineering & Biotechnology News (GEN), Bioon (生物谷), Business Standard, Drug Discovery and Development , Fierce Biotech, Health Canal, Medical News Today, Medical Xpress, MedIndia, NEWS.am, Technology.org, Today Topics, and UCR Today

Remacle, A.G., Cieplak, P., Nam, D.H., Shiryaev, S.A., Ge, X., and Strongin, A.Y.: Selective Function-Blocking Monoclonal Human Antibody Highlights the Important Role of Membrane Type-1 Matrix Metalloproteinase (MT1-MMP) in Metastasis. Oncotarget. 8:2781-2799, 2017.

Chen, L., Mulchandani, A., and Ge, X.: Spore-Displayed Enzyme Cascade with Tunable Stoichiometry. Biotechnol. Prog. 33[2]:383-389, 2017.

Nam, D.H., Fang, K., Rodriguez, C., Lopez, T., and Ge X.: Generation of Inhibitory Monoclonal Antibodies Targeting Membrane-Type 1 Matrix Metalloproteinase by Motif Grafting and CDR Optimization. Prot. Eng. Design. Select. 30[2]:113-118, 2017.

Lopez, T., Nam, D.H., Kaihara, E., Mustafa, Z., and Ge, X.: Identification of Highly Selective MMP-14 Inhibitory Fabs by Deep Sequencing. Biotechnol. Bioeng 114[6]:1140-1150, 2017.

Lee, K.B., Nam, D.H., Nuhn, J.A., Wang, J., Schneider, I.C., and Ge, X.: Direct Expression of Active Human Tissue Inhibitors of Metalloproteinases by Periplasmic Secretion in Escherichia Coli. Microb. Cell Fact. 16[1]:73, 2017.

Rodriguez, C., Nam, D.H., Kruchowy, E., and Ge, X.: Efficient Antibody Assembly in E. coli Periplasm by Chaperone Co-expression and Culture Optimization. Appl. Biochem. Biotechnol. 183[2]:520-529, 2017.

Chen, L., Holmes, M., Schaefer, E., Mulchandani, A., and Ge, X.: Highly Active Spore Biocatalyst by Self-Assembly of Co-Expressed Anchoring Scaffoldin and Multimeric Enzyme. Biotechnol. Bioeng. 115[3]:557-564, 2018.

Limsakul, P., Peng, Q., Wu, Y., Allen, M., Liang, J., Remacle, A.G., Lopez, T., Ge, X., Kay, B., Zhao, H., Strongin, A.Y., Yang, X.-L., Lu, S., and Wang, Y.: Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface. Cell Chem. Biol. 25[4]:370-379, 2018.

Chen, L., and Ge, X.: Correlation Between Size and Activity Enhancement of Recombinantly Assembled Cellulosomes. Appl. Biochem. Biotechnol. 186[4]:937-948, 2018.

Chen, K.E., Chen, C., Radecki, K.C., Bustamante, K., Lopez, T., Lorenson, M.Y., Ge, X., and Walker, A.M.: Use of a Novel Camelid-inspired Human Antibody Demonstrates the Importance of MMP-14 to Cancer Stem Cell Function in the Metastatic Process. Oncotarget. 9[50]:29431-29444, 2018.

Lopez, T., Chen, C., Ramirez, A., Chen, K.E., Lorenson, M.Y., Benitez, C., Mustafa, Z., Pham, H., Sanchez, R., Walker, A.M., and Ge, X.: Epitope Specific Affinity Maturation Improved Stability of Potent Protease Inhibitory Antibodies. Biotechnol. Bioeng. 115[11]:2673-2682, 2018.

Lopez, T., Ramirez, A., Benitez, C., Mustafa, Z., Pham, H., Sanchez, R., and Ge, X.: Selectivity Conversion of Protease Inhibitory Antibodies. Antibody Therapeutics 1[2]:55-63, 2018.

Wang, J., Boddupalli, A., Koelbl, J., Nam, D.H., Ge, X., Bratlie, K., and Schneider, I.C.: Degradation and Remodeling of Epitaxially Grown Collagen Fibrils. Cell Mol Bioeng. 12[1]:69-84, 2019.

Lee, K.B., Dunn, Z., and Ge, X.: Reducing Proteolytic Liability of an MMP-14 Inhibitory Antibody by Site-Saturation Mutagenesis. Prot. Sci. 28[3]:643-653, 2019.

Yu, X., Niks, D., Ge, X., Liu, H., Hille, R., and Mulchandani, A.: Synthesis of Formate from CO2 Gas Catalyzed by an O2-tolerant NAD-Dependent Formate Dehydrogenase and Glucose Dehydrogenase. Biochem. 58[14]:1861-1868, 2019.

Lopez, T., Mustafa, Z., Chen, C., Lee, K.B., Ramirez, A., Benitez, C., Luo, X., Ji, R.-R., and Ge, X.: Functional Selection of Protease Inhibitory Antibodies. Proc Natl Acad Sci U. S. A. 116 [33]:16314-16319, 2019.

Mei, M., Li, J., Wang, S., Lee, K.B., Iverson, B.L., Zhang, G., Ge, X., and Yi, L.: Prompting Fab Yeast Surface Display Efficiency by ER Retention and Molecular Chaperon Coexpression. Front Bioeng. Biotechnol. 7:362, 2019.

Sedki, M., Chen, X., Chen, C., Ge, X., and Mulchandani, A.: Non-lytic M13 Phage-based Highly Sensitive Impedimetric Cytosensor for Detection of Coliforms. Biosensors Bioelectronics. 148:111794, 2020.

Li, X., Zhao, Y., Chen, C., Lee, H.-H., Wang, Z., Zhang, N., Kolonin, M.G., An, Z., Ge, X., Scherer, P.E., and Sun, K.: Critical Role of Matrix Metalloproteinase 14 in Adipose Tissue Remodeling During Obesity. Mol. Cell. Biol. 40[8]:e00564-19, 2020.

Lee, K.B., Dunn, Z., Lopez, T., Mustafa, Z., and Ge, X.: Generation of Highly Selective Monoclonal Antibodies Inhibiting a Recalcitrant Protease Using Decoy Designs. Biotechnol. Bioeng. 117[12]:3664-3676, 2020.

Nam, D.H., Lee, K.B., Kruchowy, E., Pham, H., and Ge, X.: Protease Inhibition Mechanism of Camelid-Like Synthetic Human Antibodies. Biochem. 59[40]:3802-3812, 2020.

Chow, C.F.W., Guo,X., Asthana, P., Zhang, S., Wong, S.K.K., Fallah, S., Che, S., Gurung, S., Wang, Z., Lee, K.B., Ge, X., Yuan, S., Xu, H., Ip, J.P.K., Jiang, Z., Zhai, L., Wu, J., Zhang, Y., Mahato, A.K., Saarma, M., Lin, C.Y., Kwan, H.Y., Huang, T., Lyu, A., Zhou Z., Bian, Z.-X., and Wong, H.L.X.: Body Weight Regulation via MT1-MMP-Mediated Cleavage of GFRAL. Nat. Metab. 4[2]:203-212, 2022.

Cahuzac, H., Sallustrau, A., Malgorn C., Beau, F., Barbe, P., Babin, V., Dubois, S., Palazzolo, A., Thai, R., Correia, I., Li, K.B., Garcia-Argote, S., Lequin, O., Keck, M., Nozach, H., Feuillastre, S., Ge, X., Pieters, G., Audisio, D., and Devel, L. Monitoring In Vivo Performances of Protein-Drug Conjugates Using Site-Selective Dual Radiolabeling and Ex Vivo Digital Imaging. J. Med. Chem. 65[9]:6953–6968, 2022.

Guo, X., Asthana, P., Gurung, S., Zhang, S., Wong, S. K. K., Fallah, S., Chow, C. F. W., Che, S., Zhai, L., Wang, Z., Ge, X., Jiang, Z., Wu, J., Zhang, Y., Wu, X., Xu, K., Lin, C. Y., Kwan, H. Y., Lyu, A., Zhou, Z., Bian, Z.-X., Wong, H. L. X: Regulation of Age-Associated Insulin Resistance by MT1-MMP-Mediated Cleavage of Insulin Receptor. Nat. Comm. 13:3749, 2022.

Guo, X., Cao, J., Wu, J., Huang, J., Asthana, P., Wong, S.K.K., Ye, Z.-W., Gurung, S., Zhang, Y., Wang, S., Wang, Z., Ge, X., Kwan H.Y., Lu, A., Chan, K.M., Wong, N., Huang, J., Zhou, Z., Bian, Z.-X. Yuan, S., and Wong, H.L.X.: Control of SARS-CoV-2 Infection by MT1-MMP-Mediated Shedding of ACE2. Nat. Comm. 13: 7907, 2022.

Ji, Y.-B., Gao, Q., Wang, F., Tan, X.-X., Ma, Y.-Z., Chen, Y.-M., Song, D.-P., Hoo, R.L.C., Wang, Z., Ge, X., Guo, F.-Y., and Chang, J.: An MMP-9 Exclusive Neutralizing Antibody Attenuates Blood-Brain Barrier Breakdown in Mice and MMP-9 Activity in Stroke Patients. Pharmacological Res. 190:106720, 2023.

Matsuoka, Y., Furutani, K., Lee, K. B., Ge, X., and Ji, R. R.: Inhibition of Diabetic Neuropathic Pain by Intrathecal and Intravenous Administration of MMP-9 Monoclonal Antibody in Mice.J Pain.24[4]: 46, 2023.

Chen, C., Wang, Z., Kang, M., Lee, K.B., and Ge, X.: High-Fidelity Large-Diversity Monoclonal Mammalian Cell Libraries by Cell Cycle Arrested Recombinase-Mediated Cassette Exchange (aRMCE). Nat Comm. In Revision.

Choe, H., Antee, T., Ge, X.: Substrate Derived Sequences Act as Subsite Blocking Motifs in Protease Inhibitory Antibodies. Prot. Sci. In press. DOI: https://doi.org/10.1002/pro.4691

Lee, K.B., Matsuoka, Y., Wei, X., Ji, R.-R., Ge, X.: Management of Diabetic Neuropathy by Highly Selective and Potent mAb Inhibitors of MMP-9. Submitted.

Nam, D.H., Lee, K.B., and Ge, X.: Functional Production of Catalytic Domains of Human MMPs. In Proteases and Cancer – Methods in Molecular Biology book series. Editors, Santiago Cal and Alvaro J. Obaya, Springer, Clifton NJ, 1731:65-72, 2018.

Nam, D.H., and Ge, X.: Generation of Highly Selective MMP Antibody Inhibitors. In Proteases and Cancer – Methods in Molecular Biology book series. Editors, Santiago Cal and Alvaro J. Obaya, Springer, Clifton NJ, 1731:307-324, 2018.

Lee, K.B., and Ge, X.: Generation of Protease Inhibitory Antibodies by Functional In Vivo Selection. In Proteases and Cancer – Methods in Molecular Biology book series. Editor, Salvatore Santamaria, Springer, In Press.

Chilkoti, A., Carlson, K.T., Christensen, T., Filipe, C.D.M., and Ge, X.: Purification of Low-Abundant Recombinant Proteins from Cell Culture. WO 2008/024311 A3 (2008-02-28)

Ge X, Mazor Y, Georgiou G. Methods for Creating Antibody Libraries. US Patent Application 12/868,399 (2011-03-03); EP2470653A1 (2012-07-04 Application); CA2772298A1 (2011-03-03 Application); WO2011025826A1 (2011-03-03 Application)

Reddy ST, Ge X, Boutz D, Ellington AD, Marcotte EM, Georgiou G. Rapid Isolation of Monoclonal Antibodies from Animals. US Patent 9,090,674 B2 (2015-07-28); EP2572203B1 (2017-10-25); KR20130135028A (2013-12-10 Application); CN103003697A (2013-03-27 Application); CA2799746A1 (2011-11-24 Application); WO2011146514A3 (2012-11-22 Application)

Ge X, Nam DH, Lopez T. Inhibitory Antibodies and Methods of Use Thereof. US Patent 10,975,166 (2021-04-13); US20210087296A1 (2021-03-25 Publication); WO2018067198A1 (2018-04-12 Application); PCT/US2017/022341 (2017-03-14 Application); US16/223,943 (2018-12-18 Application); US20190185581A1 (2019-06-20 Publication); UC Case No. 2015-758-3

Ge X, Lee KB. Protease Inhibitory Antibodies and Method of Use Thereof. US Patent 11,332,546 (2022-05-17); US20200392251A1 (2020-12-17 Publication); US62/850,941 (2019-05-21, Application); US16/880,747 (2020-05-21, Application); UC Case No. 2019-764-2, 2021-801-1

Ge X, Lopez T, Ji RR. MMP-9 Antibodies and Methods of Use Thereof. WO/2020/237092 (2020-11-26); US62/851,001 (2019-05-21 Application); PCT/US2020/034076 (2020-05-21, Application); UC Case No. 2019-118-2