Research Interests

Inositol 1,4,5-trisphosphate receptors (IP3Rs) form a family of intracellular Ca2+ channels that integrate multiple cellular stimuli (including neurotransmitters, hormones, growth factors, odorants, and light) to release Ca2+ from intracellular stores such as the endoplasmic reticulum (ER) into the cytoplasm. IP3R channels are activated by their primary ligands, Ca2+ and IP3, and are modulated via complex interactions with numerous intracellular ligands. Due to the fundamental importance of IP3R channels in cellular Ca2+ signaling which affect many human diseases, understanding the molecular architecture of this class of integral membrane proteins is of broad interest to many different research communities. A central mechanistic question in IP3R gating is how IP3 binding in the N-terminal sequence of the channel protein is communicated to the ion conduction pore formed near the C-terminus. My aim is to get subnanometer resolution three-dimensional (3D) architecture of the full-length channel protein using single-particle electron cryomicroscopy(cryo-EM) and computational methods to reveal its biological functions in terms of its molecular architecture.

Publications

  • Fan G, Baker ML, Wang, Z, Baker MR, Sinyagovskiy PA, Chiu W, Ludtke SJ and Serysheva II. Gating Machinery of IP3R Channels Revealed by Electron Cryo-Microscopy. Nature 2015. (In press)
  • Baker MR, Fan G, Serysheva II. Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment. Eur J Transl Myol. 2015;25(1):35-48. Link
  • Shi X, Wang Z, Yan L, Ezemaduka An, Fan G, Wang R, Fu X, Yin C, Chang Z. Small heat shock protein AgsA forms dynamic fibrils. FEBS Lett. 2011 Nov 4;585(21):3396-402. Link
  • Chang Z, Wang X, Wei R, Liu Z, Shan H, Fan.G, Hu H. Functional Expression and Purification of CYP93C20 a Plant Membrane-Associated Cytochrome P450 from Medicago truncatula. Protein Expr Purif. 2010 Dec 4. Link

Education

Postdoctoral Research Fellow - UTHSC, Department of Biochemistry and Molecular Biology, Advisor:Irina Serysheva, 2012-present

Ph.D. - Peking University Health Science Center, Department of Biophysics, China, Advisor:Changcheng Yin, 2007-2012

B.S. - Shandong University, Department of Nursing, China, 2002-2007

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