AEE Services
The core contains four major functional modules:
- Lead antibody generation
- Monoclonal antibody gene cloning and humanization
- Antibody affinity maturation
- Cell line development & antibody production
List of services is shown below:
SERVICE PROVIDED |
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MONOCLONAL ANTIBODY SERVICE |
Antigen design and expression |
Monoclonal antibody production from hybridoma cell line (mouse, rat and rabbit) |
Molecular cloning of VH and VL from mouse, rat, rabbit hybridoma cell lines |
Molecular cloning VH and VL from single B cells |
ANTIBODY ENGINEERING |
scFv production in E. coli |
Full length IgG expression and purification in different isotypes (IgG1, IgG2, IgG4) in HEK293 or CHO cells |
Humanization of monoclonal antibody from mouse, rat, rabbit or other animals |
Antibody affinity maturation |
Antibody affinity determination by surface plasmon resonance (SPR) method using Biacore T-100 |
Polyclonal antibody purification |
Epitope mapping |