AEE Services

The core contains four major functional modules:

  1. Lead antibody generation
  2. Monoclonal antibody gene cloning and humanization
  3. Antibody affinity maturation
  4. Cell line development & antibody production

 

List of services is shown below:

SERVICE PROVIDED
MONOCLONAL ANTIBODY SERVICE
Antigen design and expression
Monoclonal antibody production from hybridoma cell line (mouse, rat and rabbit)
Molecular cloning of VH and VL from mouse, rat, rabbit hybridoma cell lines
Molecular cloning VH and VL from single B cells
ANTIBODY ENGINEERING
scFv production in E. coli
Full length IgG expression and purification in different isotypes (IgG1, IgG2, IgG4) in HEK293 or CHO cells
Humanization of monoclonal antibody from mouse, rat, rabbit or other animals
Antibody affinity maturation
Antibody affinity determination by surface plasmon resonance (SPR) method using Biacore T-100
Polyclonal antibody purification
Epitope mapping