AEE Services
The core contains four major functional modules:
- Lead antibody generation
- Monoclonal antibody gene cloning and humanization
- Antibody affinity maturation
- Cell line development & antibody production
List of services is shown below:
| SERVICE PROVIDED |
|---|
| MONOCLONAL ANTIBODY SERVICE |
| Antigen design and expression |
| Monoclonal antibody production from hybridoma cell line (mouse, rat and rabbit) |
| Molecular cloning of VH and VL from mouse, rat, rabbit hybridoma cell lines |
| Molecular cloning VH and VL from single B cells |
| ANTIBODY ENGINEERING |
| scFv production in E. coli |
| Full length IgG expression and purification in different isotypes (IgG1, IgG2, IgG4) in HEK293 or CHO cells |
| Humanization of monoclonal antibody from mouse, rat, rabbit or other animals |
| Antibody affinity maturation |
| Antibody affinity determination by surface plasmon resonance (SPR) method using Biacore T-100 |
| Polyclonal antibody purification |
| Epitope mapping |