Selected Publications

• Microbial Rhodopsins:  Diversity, Mechanisms, and Optogenetic Applications
  Govorunova EG, Sineshchekov OA, Li H, Spudich JL.
  Annu Rev Biochem.  2017.  86:845-872.
• Photochemical Reaction Cycle Transitions During Anion Channelrhodopsin Gating
  Sineshchekov OA, Li H, Govorunova EG, Spudich JL.
  Proc Natl Acad Sci USA.  2016.  113:E1993-2000.
• In vitro activity of a purified natural anion channelrhodopsin
  Li H, Sineshchekov OA, Wu G, Spudich JL.
  J Biol Chem. 2016 Dec 2;291(49):25319-25325. doi: 10.1074/jbc.C116.760041. Epub 2016 Oct 27.
  PMID: 27789708
• Structurally Distinct Cation Channelrhodopsins from Cryptophyte Algae
  Govorunova EG, Sineshchekov OA, Spudich JL.
  Biophys J. 2016 Jun 7;110(11):2302-4. doi: 10.1016/j.bpj.2016.05.001. Epub 2016 May 24.
  PMID: 27233115
• Resonance Raman Study of an Anion Channelrhodopsin: Effects of Mutations near the Retinylidene Schiff Base
  Yi A, Mamaeva N, Li H, Spudich JL, Rothschild KJ.
  Biochemistry. 2016 Apr 26;55(16):2371-80. doi: 10.1021/acs.biochem.6b00104. Epub 2016 Apr 14.
  PMID: 27039989
• Photochemical Reaction Cycle Transitions During Anion Channelrhodopsin Gating
  Sineshchekov OA, Li H, Govorunova EG, Spudich JL.
  Proc Natl Acad Sci USA. 2016 Mar 21. pii: 201525269. [Epub ahead of print]
  PMID: 27001860
• Natural Light-gated Anion Channels:  A Family of Microbial Rhodopsins for Advanced Optogenetics
  Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL.
  Science.  2015.  349:647-650.
  PMID: 26113638
• Proteomonas sulcata ACR1: A fast anion channelrhodopsin
  Govorunova EG, Sineshchekov OA, Spudich JL.
  Photochem Photobiol. 2015 Dec 20. doi: 10.1111/php.12558. [Epub ahead of print]
  PMID: 26686819
• Gating Mechanisms of a Natural Anion Channelrhodopsin
  Sineshchekov OA, Govorunova EG, Li H, Spudich JL.
  Proc Natl Acad Sci USA. 2015 Nov 17;112(46):14236-41. doi: 10.1073/pnas.1513602112. Epub 2015 Nov 2.
  PMID: 26578767
• In Vitro Demonstration of Dual Light-Driven Na(+)/H(+) Pumping by a Microbial Rhodopsin
  Li H, Sineshchekov OA, da Silva GF, Spudich JL.
  Biophys J. 2015 Oct 6;109(7):1446-53. doi: 10.1016/j.bpj.2015.08.018.
  PMID: 26445445
• Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics
  Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL.
  Science. 2015 Aug 7;349(6248):647-50. doi: 10.1126/science.aaa7484. Epub 2015 Jun 25.
  PMID: 26113638
• Cation-specific conformations in a dual-function ion-pumping microbial rhodopsin.
  da Silva GF, Goblirsch BR, Tsai AL, Spudich JL.
  Biochemistry. 2015 Jun 30;54(25):3950-9. doi: 10.1021/bi501386d. Epub 2015 Jun 17.
  PMID: 26037033
• Platymonas subcordiformis Channelrhodopsin-2 Function. Part I: The photochemical reaction cycle.
  Szundi I, Li H, Chen E, Bogomolni R, Spudich JL, Kliger DS.
  J Biol Chem. 2015 Jul 3;290(27):16573-84. doi: 10.1074/jbc.M114.631614. Epub 2015 May 13.
  PMID: 25971972
• Proton transfers in a channelrhodopsin-1 studied by Fourier transform infrared (FTIR) difference spectroscopy and site-directed mutagenesis.
  Ogren JI, Yi A, Mamaev S, Li H, Spudich JL, Rothschild KJ.
  J Biol Chem. 2015 May 15;290(20):12719-30. doi: 10.1074/jbc.M114.634840. Epub 2015 Mar 23.
  PMID: 25802337
• Comparison of the structural changes occurring during the primary phototransition of two different channelrhodopsins from Chlamydomonas algae.
  Ogren JI, Yi A, Mamaev S, Li H, Lugtenburg J, DeGrip WJ, Spudich JL, Rothschild KJ.
  Biochemistry. 2015 Jan 20;54(2):377-88. doi: 10.1021/bi501243y. Epub 2014 Dec 18.
  PMID: 25469620
• Channelrhodopsin photochromic reactions provide multicolor optogenetic control.
  Spudich JL.
  Biophys J. 2014 Oct 7;107(7):1489-90. doi: 10.1016/j.bpj.2014.08.021.
  PMID: 25296299
• His166 is the schiff base proton acceptor in attractant phototaxis receptor sensory rhodopsin I.
  Sasaki J, Takahashi H, Furutani Y, Sineshchekov OA, Spudich JL, Kandori H.
  Biochemistry. 2014 Sep 23;53(37):5923-9. doi: 10.1021/bi500831n. Epub 2014 Sep 8.
  PMID: 25162914
• Retinal chromophore structure and schiff base interactions in red-shifted channelrhodopsin-1 from Chlamydomonas augustae.
  Ogren JI, Mamaev S, Russano D, Li H, Spudich JL, Rothschild KJ.
  Biochemistry. 2014 Jun 24;53(24):3961-70. doi: 10.1021/bi500445c. Epub 2014 Jun 16.
  PMID: 24869998
• Role of a helix B lysine residue in the photoactive site in channelrhodopsins.
  Li H, Govorunova EG, Sineshchekov OA, Spudich JL.
  Biophys J. 2014 Apr 15;106(8):1607-17. doi: 10.1016/j.bpj.2014.03.002.
  PMID: 24739160
• A molecular voltmeter based on fluorescence dynamics.
  Spudich JL.
  Biophys J. 2014 Feb 4;106(3):497-9. doi: 10.1016/j.bpj.2013.12.029. No abstract available.
  PMID: 24507590
• Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes.
  Ran T, Ozorowski G, Gao Y, Sineshchekov OA, Wang W, Spudich JL, Luecke H.
  Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1965-80. doi: 10.1107/S0907444913017575. Epub 2013 Sep 20.
  PMID: 24100316
• Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis.
  Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL.
  J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30.
  PMCID: PMC3795289
• Mechanism divergence in microbial rhodopsins.
  Spudich JL, Sineshchekov OA, Govorunova EG.
  Biochim Biophys Acta. 2013 Jul 3. doi:pii: S0005-2728(13)00103-5. 10.1016/j.bbabio.2013.06.006.
  PMCID: PMC3844102