Selected Publications

• Microbial Rhodopsins:  Diversity, Mechanisms, and Optogenetic Applications (external link)
  Govorunova EG, Sineshchekov OA, Li H, Spudich JL.
  Annu Rev Biochem.  2017.  86:845-872.
• Photochemical Reaction Cycle Transitions During Anion Channelrhodopsin Gating (external link)
  Sineshchekov OA, Li H, Govorunova EG, Spudich JL.
  Proc Natl Acad Sci USA.  2016.  113:E1993-2000.
• In vitro activity of a purified natural anion channelrhodopsin (external link)
  Li H, Sineshchekov OA, Wu G, Spudich JL.
  J Biol Chem. 2016 Dec 2;291(49):25319-25325. doi: 10.1074/jbc.C116.760041. Epub 2016 Oct 27.
  PMID: 27789708
• Structurally Distinct Cation Channelrhodopsins from Cryptophyte Algae (external link)
  Govorunova EG, Sineshchekov OA, Spudich JL.
  Biophys J. 2016 Jun 7;110(11):2302-4. doi: 10.1016/j.bpj.2016.05.001. Epub 2016 May 24.
  PMID: 27233115
• Resonance Raman Study of an Anion Channelrhodopsin: Effects of Mutations near the Retinylidene Schiff Base (external link)
  Yi A, Mamaeva N, Li H, Spudich JL, Rothschild KJ.
  Biochemistry. 2016 Apr 26;55(16):2371-80. doi: 10.1021/acs.biochem.6b00104. Epub 2016 Apr 14.
  PMID: 27039989
• Photochemical Reaction Cycle Transitions During Anion Channelrhodopsin Gating (external link)
  Sineshchekov OA, Li H, Govorunova EG, Spudich JL.
  Proc Natl Acad Sci USA. 2016 Mar 21. pii: 201525269. [Epub ahead of print]
  PMID: 27001860
• Natural Light-gated Anion Channels:  A Family of Microbial Rhodopsins for Advanced Optogenetics (external link)
  Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL.
  Science.  2015.  349:647-650.
  PMID: 26113638
• Proteomonas sulcata ACR1: A fast anion channelrhodopsin (external link)
  Govorunova EG, Sineshchekov OA, Spudich JL.
  Photochem Photobiol. 2015 Dec 20. doi: 10.1111/php.12558. [Epub ahead of print]
  PMID: 26686819
• Gating Mechanisms of a Natural Anion Channelrhodopsin (external link)
  Sineshchekov OA, Govorunova EG, Li H, Spudich JL.
  Proc Natl Acad Sci USA. 2015 Nov 17;112(46):14236-41. doi: 10.1073/pnas.1513602112. Epub 2015 Nov 2.
  PMID: 26578767
• In Vitro Demonstration of Dual Light-Driven Na(+)/H(+) Pumping by a Microbial Rhodopsin (external link)
  Li H, Sineshchekov OA, da Silva GF, Spudich JL.
  Biophys J. 2015 Oct 6;109(7):1446-53. doi: 10.1016/j.bpj.2015.08.018.
  PMID: 26445445
• Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics (external link)
  Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL.
  Science. 2015 Aug 7;349(6248):647-50. doi: 10.1126/science.aaa7484. Epub 2015 Jun 25.
  PMID: 26113638
• Cation-specific conformations in a dual-function ion-pumping microbial rhodopsin. (external link)
  da Silva GF, Goblirsch BR, Tsai AL, Spudich JL.
  Biochemistry. 2015 Jun 30;54(25):3950-9. doi: 10.1021/bi501386d. Epub 2015 Jun 17.
  PMID: 26037033
• Platymonas subcordiformis Channelrhodopsin-2 Function. Part I: The photochemical reaction cycle. (external link)
  Szundi I, Li H, Chen E, Bogomolni R, Spudich JL, Kliger DS.
  J Biol Chem. 2015 Jul 3;290(27):16573-84. doi: 10.1074/jbc.M114.631614. Epub 2015 May 13.
  PMID: 25971972
• Proton transfers in a channelrhodopsin-1 studied by Fourier transform infrared (FTIR) difference spectroscopy and site-directed mutagenesis. (external link)
  Ogren JI, Yi A, Mamaev S, Li H, Spudich JL, Rothschild KJ.
  J Biol Chem. 2015 May 15;290(20):12719-30. doi: 10.1074/jbc.M114.634840. Epub 2015 Mar 23.
  PMID: 25802337
• Comparison of the structural changes occurring during the primary phototransition of two different channelrhodopsins from Chlamydomonas algae. (external link)
  Ogren JI, Yi A, Mamaev S, Li H, Lugtenburg J, DeGrip WJ, Spudich JL, Rothschild KJ.
  Biochemistry. 2015 Jan 20;54(2):377-88. doi: 10.1021/bi501243y. Epub 2014 Dec 18.
  PMID: 25469620
• Channelrhodopsin photochromic reactions provide multicolor optogenetic control. (external link)
  Spudich JL.
  Biophys J. 2014 Oct 7;107(7):1489-90. doi: 10.1016/j.bpj.2014.08.021.
  PMID: 25296299
• His166 is the schiff base proton acceptor in attractant phototaxis receptor sensory rhodopsin I. (external link)
  Sasaki J, Takahashi H, Furutani Y, Sineshchekov OA, Spudich JL, Kandori H.
  Biochemistry. 2014 Sep 23;53(37):5923-9. doi: 10.1021/bi500831n. Epub 2014 Sep 8.
  PMID: 25162914
• Retinal chromophore structure and schiff base interactions in red-shifted channelrhodopsin-1 from Chlamydomonas augustae. (external link)
  Ogren JI, Mamaev S, Russano D, Li H, Spudich JL, Rothschild KJ.
  Biochemistry. 2014 Jun 24;53(24):3961-70. doi: 10.1021/bi500445c. Epub 2014 Jun 16.
  PMID: 24869998
• Role of a helix B lysine residue in the photoactive site in channelrhodopsins. (external link)
  Li H, Govorunova EG, Sineshchekov OA, Spudich JL.
  Biophys J. 2014 Apr 15;106(8):1607-17. doi: 10.1016/j.bpj.2014.03.002.
  PMID: 24739160
• A molecular voltmeter based on fluorescence dynamics. (external link)
  Spudich JL.
  Biophys J. 2014 Feb 4;106(3):497-9. doi: 10.1016/j.bpj.2013.12.029. No abstract available.
  PMID: 24507590
• Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes. (external link)
  Ran T, Ozorowski G, Gao Y, Sineshchekov OA, Wang W, Spudich JL, Luecke H.
  Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1965-80. doi: 10.1107/S0907444913017575. Epub 2013 Sep 20.
  PMID: 24100316
• Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis. (external link)
  Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL.
  J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30.
  PMCID: PMC3795289
• Mechanism divergence in microbial rhodopsins. (external link)
  Spudich JL, Sineshchekov OA, Govorunova EG.
  Biochim Biophys Acta. 2013 Jul 3. doi:pii: S0005-2728(13)00103-5. 10.1016/j.bbabio.2013.06.006.
  PMCID: PMC3844102