Equipment

Nikon N-STORM Super-Resolution Microscope System with A1Rsi Confocal and TIRF

Nikon A1Rsi Confocal & STORM microscope
This new Nikon n-STORM Super-Resolution A1R HD resonant scanner confocal system combines ultra-high speeds and enhanced sensitivity.
New HD components allow for 1K x 1K fast imaging with the resonant scanner. The improved electronics provide greater processing power for cleaner images at high speeds.Stochastic Optical Reconstruction Microscopy (STORM) results in high accuracy localization to create images with spatial resolution up to 20nm (10-fold over conventional optical microscopy).
Capable of imaging in both 2D and 3D modes.Multi-detector unit with 2 channel GaAsp Spectral Detector to give brighter signals, enhanced signal-to-noise, allow less phototoxicity while enabling spectral image acquisition.
Total Internal Reflection Fluorescence (TIRF) microscopy capabilities.

 

Key features include:

6 laser lines

Objectives

  • 405 nm LD 20 mW
  • 445 nm LD 20 mW
  • 488 nm DPSS 70mW
  • 514 nm DPSS 40mW
  • 561 nm DPSS 70mW
  • 647 nm LD 125 mW
  • CFI PLAN APO LAMBDA 4X
  • CFI PLAN APO LAMBDA 10X
  • CFI PLAN APO LAMBDA 20X
  • CFI PLAN FLUOR 40X OIL
  • CFI HP APO TIRF AC 100X OIL


Nikon N-SIM Structured Illumination Super-Resolution Microscope System

Nikon SIM microscope
The system uses Structured Illumination technology, which gives a two-fold enhancement in resolution (over conventional optical microscopy).
Has ability to image dynamic events in live samples in both 2D, 3D and TIRF-SIM (higher signal-to-noise to enhance resolution even further).
Includes top of the line super-resolution and TIRF objectives (100X) have superior optics with the least amount of lens defects/aberrations.
A wide-ranging illumination and detection systems to give users greater flexibility.

Key features include:

6 laser lines Objectives
  • 405 nm LD 20 mW
  • 445 nm LD 20 mW
  • 488 nm DPSS 70mW
  • 514 nm DPSS 20 mW
  • 561 nm DPSS 70mW
  • 647 nm LD 125 mW
  • CFI PLAN APO LAMBDA 10X
  • CFI PLAN APO LAMBDA 20X
  • CFI HP APO TIRF AC 100X OIL


Nikon A1R Confocal Laser Microscope System

picture of NikonA1R
The Nikon A1R is a modular confocal system consisting of a separate scan head, standard 4 PMT detector module plus an additional PMT for pseudo-DIC imaging, control module, laser module, and spectral detector module. The A1R has a standard high-resolution confocal mode up to 4096 x 4096 pixels as well as a high speed resonant mode capable of scanning 230 frames per sec at 512 x 64 pixels. In addition to laser-based, confocal image acquisition, this Nikon platform also utilizes a high-end digital camera for non-confocal image acquisition.

The updated A1R has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise ratio, and less phototoxicity.

The updated A1R has the ability to perform Enhanced Resolution algorithms on point scanning confocal datasets to further increase resolution of images, and also Retirement/Replacement of Confocal Workstations allows for future expansion and faster processing, including GPU 3D Rendering.

 

Key features include:

6 laser lines Objectives
  • 405 nm LD 20 mW
  • 445 nm LD 20 mW
  • 488 nm DPSS 70mW
  • 514 nm DPSS 40 mW
  • 561 nm DPSS 70mW
  • 640 nm LD 125 mW
  • 10X Plan-Fluor/0.3 NA w/Phase
  • 20X Plan-Apo/0.75 NA
  • 40X Plan-Fluor/0.75 NA
  • 40X Plan-Fluor/1.3 NA Oil
  • 60X Apo TIRF/1.49 NA Oil
  • 100X Plan-Apo/1.4 NA Oil



 

Nikon A1 Confocal Laser Microscope System + PicoQuant

picture of Nikon A1 Confocal Laser Microscope System & PicoQuant
The Nikon A1 is a basic confocal system consisting of a separate scan head, standard 5 PMT detectors, control module, and laser module. The A1 has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise, and allow less phototoxicity.  High-resolution confocal mode permits up to 4096 X 4096 pixels. This laser scanning confocal system is similar to that of the Nikon A1R in that it is configured on the Nikon TiE Widefield, inverted, fluorescence microscope. NIS Elements imaging software is used to control acquisition and analysis.

The PicoQuant Imaging System is an upgrade package added to the Nikon A1 system for the purpose of performing Fluorescence Lifetime Imaging (FLIM). This system employs pulsed lasers for the purpose of assessing protein-protein interactions and is unique to the IBP Imaging Core. The system is equipped with 439 nm (121 mW peak power) and 483 nm (136 Mw peak power) lasers.

  • 5 PMTs (photomultiplier tubes)
  • Autofocus system
  • Nikon TiE Wide field fluorescence microscope

 

Key features include:

4 laser lines Objectives
  • 405 nm diode at 100 mW
  • 488 nm diode at 350 mW
  • 561 nm diode at 480 mW
  • 640 nm diode at 100 mW
  • 10X Plan-Fluor/0.3 NA w/ phase
  • 20X Plan-Apo/0.75 NA
  • 40X Plan-Fluor/0.75 NA
  • 40X Plan-Fluor/1.3 NA Oil
  • 60X PApo TIRF/1.49 NA Oil
  • 100X Plan-Apo/1.4 NA Oil



Lambert LIFA Fluorescence Lifetime Imaging Microscopy System

Nikon A1Rsi Confocal & STORM microscope
The Lambert FLIM system is a fully developed system for frequency domain measurement of fluorescence lifetime with high accuracy, stability and acquisition/processing speed.

 

Key features include:

Nikon TiE Widefield fluorescence microscope
High power multi-LED unit with peak emissions at: Objectives
  • 405nm (BFP)
  • 447nm (CFP)
  • 479nm (GFP)
  • 532nm (RFP)
  • 10X Plan-Fluor/0.45 NA w/phase
  • 20X Plan-Apo/0.75 NA
  • 40X Plan-Fluor/1.30 NA Oil
  • 60X Plan-Apo/1.40 NA Oil



Calcium Rig

Picture of Calcium Rig
The Nikon TiE Imaging System is a high resolution system designed for fluorescence imaging of small molecules in living cells. It is a filter-based, wide-field fluorescence system designed for measurement of intracellular signaling molecules including calcium ions, hydrogen ions (pH), nitric oxide, and other small molecules using appropriate fluorophores. Nikon’s NIS Elements imaging software is used to control acquisition and analysis of captured images. The software features include capabilities for single wavelength, multiple wavelength, and ratiometric mode fluorescence imaging.

 

Key features include:

6 laser lines Objectives
  • 405 nm LD 20 mW
  • 445 nm LD 20 mW
  • 488 nm DPSS 70mW
  • 514 nm DPSS 40 mW
  • 561 nm DPSS 70mW
  • 647 nm LD 125 mW
  • CFI PLAN APO LAMBDA 4X
  • CFI PLAN APO LAMBDA 10X
  • CFI PLAN APO LAMBDA 20X
  • CFI PLAN FLUOR 40X OIL NA1.30 WD 0.20MM
  • CFI HP APO TIRF AC 100X OIL



IVIS LUMINA

picture of IVIS Lumina XR
The IVIS Lumina XR is live, whole animal imaging system capable of performing longitudinal fluorescence, bioluminescence and X-ray scans on in vivo and ex vivo subjects. The IVIS Lumina XR is capable of imaging all common fluorescent and bioluminescent reporters/dyes ranging from green (~488 nm) to near-infrared. The unit also contains an isoflurane-based, gas anesthesia system used for live animal studies, though investigators may utilize other methods of anesthesia provided they are approved and documented by the UT-IACUC.

 

Key features include:

IVIS Lumina XR X-Ray Module
  • High-sensitivity in vivo and in vitro imaging of fluorescence and bioluminescence
  • Back-thinned, back-illuminated grade 1 CCD provides high quantum efficiency over the entire visible to near-infrared spectrum
  • Light-tight imaging chamber
  • 8 position emission filter wheels
  • 10 position excitation filter wheels
  • 5 filter wheel choices for a broad range of fluorescence applications
  • LED lamps for photographic images
  • Heated stage to maintain optimum body temperature
  • Automated motor controlled stage, filter wheel, lens position, and f-stop
  • Average X-ray acquisition in less than 10 seconds
  • Radiation shielded Cabinet
  • Exceeds standards set by the US FDA Center for Devices and Radiological Health (21 CFR-1020.40)
  • Automated image integration to overlay with Bioluminescence, Fluorescence and Photograph
optical zoom lens attachment for close up and high-resolution X-ray



Data Processing Computers:

Workstation 1: Nikon Advanced Super-Resolution Image Analysis
picture of the data processing computer
Workstation 2: General Image Analysis:
picture of Data processing computer
NIS Elements AR; Meta Morph; LIFA-FLIM; Living Image; Image J; Slidebook; AutoQuant, and Adobe Photoshop elements 2.0.