SAM (Significance Analysis of Microarray) Results

Description of the problem:

Number of classes: 2
Number of genes: 12776
Number of genes that passed filtering criteria: 8324
Type of test used: SAM – Significance analysis of Microarray
Column of the Experiment Descriptors sheet that defines class variable : GROUP

Genes which discriminate among classes:

Table 1
There are 166 genes significant by SAM
The median false discovery rate among the 166 significant genes is 0.08539
The delta value used to identify the significant genes is 0.59305
The fudge factor for standard deviation is computed as 0.03459

Geometric mean of ratios
( Disease / Normal )
Qiagen oligo ID Description GB acc UG cluster Gene symbol
1 1.553 H002655_01 Collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive) L02870 (external link) 1640 COL7A1 (external link)
2 1.453 H007688_01 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 AF109735 (external link) 195471 PFKFB3 (external link)
3 1.757 H002994_01 Collagen, type XVIII, alpha 1 AF018081 (external link) 78409 COL18A1 (external link)
4 1.689 H016341_01 Platelet derived growth factor C NM_016205 (external link) 43080 PDGFC (external link)
5 1.422 H006183_01 Metallothionein 1X X65607 (external link) 278462 MT1X (external link)
6 1.251 H011854_01 Heterogeneous nuclear
ribonucleoprotein C (C1/C2)
M16342 (external link) 182447 HNRPC (external link)
7 1.352 H000498_01 Desmoplakin (DPI, DPII) AL031058 (external link) 74316 DSP (external link)
8 1.249 H003776_01 Aldehyde dehydrogenase 2 family
(mitochondrial)
X05409 (external link) 195432 ALDH2 (external link)
9 1.436 H012791_01 Metallothionein 1A (functional) K01383 (external link) 203967 MT1A (external link)
10 1.314 H003383_01 Ras-related C3 botulinum toxin
substrate 2 (rho family, small GTP binding protein Rac2)
Z82188 (external link) 173466 RAC2 (external link)
11 1.66 H015187_01 Homo sapiens clone N97
immunoglobulin heavy chain variable region mRNA, partial CDS
AF103295 (external link) 283882
12 1.483 H008423_01 EH-domain containing 3 NM_014600 (external link) 87125 EHD3 (external link)
13 1.333 H007009_01 Fibroblast growth factor
receptor-like 1
AK000530 (external link) 193326 FGFRL1 (external link)
14 1.23 H000238_01 KRAB associated protein 1 X97548 (external link) 228059 TIF1B (external link)
15 1.386 H011703_01 Hypothetical protein FLJ20364 AK000371 (external link) 32471 FLJ20364 (external link)
16 1.292 H014948_01 Phosphatidylglycerophosphate
Synthase
NM_024419 (external link) 278682 PGS1 (external link)
17 1.375 H006492_01 Homo sapiens CD44 isoform RC
(CD44) mRNA, complete CDS
AF098641 (external link) 306278
18 1.374 H012664_01 metallothionein 1B (functional) M13485 (external link) 211924 MT1B (external link)
19 1.292 H006579_01 Hypothetical protein FLJ10292 AK001154 (external link) 104650 FLJ10292 (external link)
20 1.427 H008064_01 KIAA0008 gene product D13633 (external link) 77695 KIAA0008 (external link)
21 1.255 H012398_01 KIAA1118 protein AB029041 (external link) 209646 KIAA1118 (external link)
22 1.451 H003725_01 Cartilage oligomeric matrix
protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)
AC003107 (external link) 1584 COMP (external link)
23 1.211 H011172_01 Heat shock protein 75 NM_016292 (external link) 182366 TRAP1 (external link)
24 1.243 H009594_01 Neuropathy target esterase AJ004832 (external link) 5038 NTE (external link)
25 1.445 H008685_01 Metallothionein 2A V00594 (external link) 118786 MT2A (external link)
26 1.279 H007583_01 Kinesin family member 4A AF071592 (external link) 279766 KIF4A (external link)
27 1.367 H013251_01 KIAA1329 protein AB037750 (external link) 21061 KIAA1329 (external link)
28 1.189 H015295_01 Homo sapiens HSPC337 mRNA,
partial CDS
AF161451 (external link) 284295
29 1.5 H011653_01 Vanilloid receptor-like protein NM_016113 (external link) 300686 VRL (external link)
30 1.405 H012717_01 Metallothionein 1F (functional) M10943 (external link) 203936 MT1F (external link)
31 1.193 H003987_01 PRO0659 protein NM_014138 (external link) 6451 PRO0659 (external link)
32 1.415 H009923_01 Vanilloid receptor-like protein 1 NM_015930 (external link) 279746 VRL-1 (external link)
33 1.313 H016122_01 TMTSP for transmembrane molecule
with thrombospondin module
NM_018676 (external link) 325667 LOC55901 (external link)
34 1.244 H012710_01 Hepatitis delta
antigen-interacting protein A
U63825 (external link) 66713 DIPA (external link)
35 0.621 H003528_01 Decay accelerating factor for
complement (CD55, Cromer blood group system)
M30142 (external link) 1369 DAF (external link)
36 0.548 H003827_01 Serum/glucocorticoid regulated
kinase
AJ000512 (external link) 296323 SGK (external link)
37 0.548 H016371_01 Hypothetical protein FLJ21212 NM_024642 (external link) 47099 FLJ21212 (external link)
38 0.584 H009347_01 Neuronal cell adhesion molecule AB002341 (external link) 7912 NRCAM (external link)
39 0.465 H001509_01 Aldo-keto reductase family 1,
member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
D17793 (external link) 78183 AKR1C3 (external link)
40 0.517 H003858_01 Aldo-keto reductase family 1,
member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-a
U05598 (external link) 201967 AKR1C2 (external link)
41 0.62 H010655_01 Hypothetical protein FLJ20546 AK000953 (external link) 279896 FLJ20546 (external link)
42 0.811 H005438_01 Homo sapiens mRNA; cDNA DKFZp434B1620 (from clone DKFZp434B1620) AL137548 (external link) 43112
43 0.728 H004078_01 Heme-binding protein NM_015987 (external link) 108675 HEBP (external link)
44 0.708 H002860_01 Inositol
polyphosphate-1-phosphatase
L08488 (external link) 32309 INPP1 (external link)
45 0.768 H002574_01 Alcohol dehydrogenase 5 (class
III), chi polypeptide
M81118 (external link) 78989 ADH5 (external link)
46 0.751 H004767_01 Tetraspan 3 AK001326 (external link) 100090 TSPAN-3 (external link)
47 0.629 H010640_01 Homo sapiens mRNA; cDNA
DKFZp434B1272 (from clone DKFZp434B1272); partial CDS
AL162032 (external link) 158258
48 0.689 H000959_01 Glycophorin C (Gerbich blood
group)
NM_002101 (external link) 81994 GYPC (external link)
49 0.721 H003965_01 Cellular repressor of
E1A-stimulated genes
AF084523 (external link) 5710 CREG (external link)
50 0.724 H002462_01 Receptor tyrosine kinase-like
orphan receptor 1
M97675 (external link) 274243 ROR1 (external link)
51 0.664 H007733_01 Retinal short-chain
dehydrogenase/reductase retSDR3
NM_016246 (external link) 18788 LOC51171 (external link)
52 0.771 H016494_01 Hypothetical protein FLJ12436 NM_024661 (external link) 69485 FLJ12436 (external link)
53 0.742 H008089_01 KIAA0469 gene product AB007938 (external link) 7764 KIAA0469 (external link)
54 0.631 H007034_01 Histamine N-methyltransferase U44111 (external link) 81182 HNMT (external link)
55 0.706 H000410_01 A kinase (PRKA) anchor protein
(gravin) 12
U81607 (external link) 788 AKAP12 (external link)
56 0.702 H001356_01 KIAA0537 gene product AB011109 (external link) 200598 KIAA0537 (external link)
57 0.538 H001341_01 Platelet-derived growth factor
receptor-like
D37965 (external link) 170040 PDGFRL (external link)
58 0.815 H002021_01 V-maf musculoaponeurotic
fibrosarcoma (avian) oncogene family, protein G
AF059195 (external link) 252229 MAFG (external link)
59 0.592 H010453_01 3-alpha hydroxysteroid
dehydrogenase type IIb
NM_016253 279801 LOC51708 (external link)
60 0.685 H005634_01 Solute carrier family 11
(proton-coupled divalent metal ion transporters), member 3
NM_014585 (external link) 5944 SLC11A3 (external link)
61 0.68 H008745_01 Acid sphingomyelinase-like
phosphodiesterase
AK000184 (external link) 42945 ASM3A (external link)
62 0.704 H003219_01 Glutathione S-transferase M3
(brain)
AF043105 (external link) 2006 GSTM3 (external link)
63 0.773 H003951_01 Predicted osteoblast protein D87120 (external link) 29882 GS3786 (external link)
64 0.735 H001047_01 Nidogen 2 AB009799 (external link) 82733 NID2 (external link)
65 0.792 H002382_01 Limbic system-associated membrane
protein
U41901 (external link) 26479 LSAMP (external link)
66 0.623 H016583_01 1,2-alpha-mannosidase IC NM_020379 (external link) 8910 HMIC (external link)
67 0.742 H011191_01 Lipin 2 D87436 (external link) 166318 LPIN2 (external link)
68 0.803 H002185_01 Niemann-Pick disease, type C1 AF002020 (external link) 76918 NPC1 (external link)
69 0.775 H000509_01 Neural precursor cell expressed,
developmentally down-regulated 4
D42055 (external link) 1565 NEDD4 (external link)
70 0.786 H007419_01 Progesterone membrane binding
protein
AJ002030 (external link) 9071 PMBP (external link)
71 0.774 H002121_01 Paraoxonase 2 AF001601 (external link) 169857 PON2 (external link)
72 0.805 H008087_01 HSPC019 protein NM_014028 (external link) 163724 HSPC019 (external link)
73 0.696 H002393_01 Sema domain, immunoglobulin
domain (Ig), short basic domain, secreted, (semaphorin) 3C
AB000220 (external link) 171921 SEMA3C (external link)
74 0.785 H014975_01 Homo sapiens mRNA; cDNA
DKFZp564N1272 (from clone DKFZp564N1272); complete CDS
AF087847 (external link) 282654
75 0.614 H001215_01 Pentaxin-related gene, rapidly
induced by IL-1 beta
M31166 (external link) 2050 PTX3 (external link)
76 0.756 H002423_01 Vascular endothelial growth
factor B
U43368 (external link) 78781 VEGFB (external link)
77 0.768 H010087_01 Destrin (actin depolymerizing
factor)
S65738 (external link) 82306 ADF (external link)
78 0.742 H000025_01 Neutral sphingomyelinase
(N-SMase) activation associated factor
X96586 (external link) 78687 NSMAF (external link)
79 0.744 H005560_01 WAS protein family, member 1 D87459 (external link) 75850 WASF1 (external link)
80 0.786 H014051_01 Very long-chain acyl-CoA
synthetase homolog 2
NM_012254 (external link) 111401 VLCS-H2 (external link)
81 0.644 H004741_01 Fibroblast growth factor 13 AF100143 (external link) 6540 FGF13 (external link)
82 0.764 H001605_01 Annexin A4 NM_001153 (external link) 77840 ANXA4 (external link)
83 0.71 H016019_01 Duodenal cytochrome b AL136693 (external link) 31297 FLJ23462 (external link)
84 0.804 H001346_01 Inositol(myo)-1(or
4)-monophosphatase 2
NM_014214 (external link) 5753 IMPA2 (external link)
85 0.635 H010918_01 WNT1 inducible signaling pathway
protein 2
AF100780 (external link) 194679 WISP2 (external link)
86 0.791 H001101_01 CD164 antigen, sialomucin D14043 (external link) 43910 CD164 (external link)
87 0.787 H004431_01 Rag C protein AF131773 (external link) 110950 GTR2 (external link)
88 0.792 H012626_01 Beta-2-microglobulin AB021288 (external link) 75415 B2M (external link)
89 0.805 H002819_01 Protective protein for
beta-galactosidase (galactosialidosis)
AL008726 (external link) 118126 PPGB (external link)
90 0.684 H002497_01 Complement component 1, r
subcomponent
M14058 (external link) 1279 C1R (external link)
91 0.691 H006676_01 Lung type-I cell
membrane-associated glycoprotein
AF030428 (external link) 135150 T1A-2 (external link)
92 0.653 H012777_01 KIAA1199 protein AB033025 (external link) 50081 KIAA1199 (external link)
93 0.721 H003789_01 Colony stimulating factor 1
(macrophage)
M37435 (external link) 173894 CSF1 (external link)
94 0.849 H008439_01 SCO (cytochrome oxidase
deficient, yeast) homolog 1
AF026852 (external link) 14511 SCO1 (external link)
95 0.774 H008994_01 Solute carrier family 31 (copper
transporters), member 2
U83461 (external link) 24030 SLC31A2 (external link)
96 0.774 H010497_01 Interferon induced transmembrane
protein 3 (1-8U)
X57352 (external link) 182241 IFITM3 (external link)
97 0.762 H011175_01 Opsin 3 (encephalopsin) NM_014322 (external link) 279926 OPN3 (external link)
98 0.701 H011671_01 Short-chain
dehydrogenase/reductase 1
AF061741 (external link) 17144 SDR1 (external link)
99 0.767 H002804_01 Prion protein (p27-30)
(Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome,
fatal fami
U29185 (external link) 74621 PRNP (external link)
100 0.792 H002258_01 UDP-glucose pyrophosphorylase 2 U27460 (external link) 77837 UGP2 (external link)
101 0.659 H001491_01 Cyclin T2 AF048731 (external link) 155478 CCNT2 (external link)
102 0.762 H002746_01 TNF receptor-associated factor 5 AB000509 (external link) 29736 TRAF5 (external link)
103 0.766 H013070_01 Sarcoglycan, epsilon AJ000534 (external link) 110708 SGCE (external link)
104 0.761 H001062_01 N-acylsphingosine amidohydrolase
(acid ceramidase)
U70063 (external link) 75811 ASAH (external link)
105 0.814 H002694_01 Glutathione peroxidase 4
(phospholipid hydroperoxidase)
X71973 (external link) 2706 GPX4 (external link)
106 0.774 H011917_01 Sorting nexin 3 AF034546 (external link) 12102 SNX3 (external link)
107 0.754 H006813_01 Homo sapiens cDNA FLJ11309 fis,
clone PLACE1010076
AK002171 (external link) 28005
108 0.821 H002793_01 Antigen identified by monoclonal
antibodies 12E7, F21 and O13
M16279 (external link) 177543 MIC2 (external link)
109 0.844 H000474_01 Testis-specific kinase 1 D50863 (external link) 79358 TESK1 (external link)
110 0.731 H000930_01 Low density lipoprotein receptor
(familial hypercholesterolemia)
L00352 (external link) 213289 LDLR (external link)
111 0.802 H009135_01 Electron-transferring-flavoprotein
dehydrogenase
S69232 (external link) 323468 ETFDH (external link)
112 0.767 H008544_01 Atrophin-1 interacting protein 1;
activin receptor interacting protein 1
AB014605 (external link) 22599 KIAA0705 (external link)
113 0.789 H000400_01 Growth arrest-specific 6 L13720 (external link) 78501 GAS6 (external link)
114 0.762 H016469_01 MEK partner 1 NM_021970 (external link) 6361 MP1 (external link)
115 0.731 H002820_01 Ectodermal-neural cortex (with
BTB-like domain)
AF059611 (external link) 104925 ENC1 (external link)
116 0.75 H016592_01 Hypothetical protein FLJ12816 NM_022060 (external link) 9175 FLJ12816 (external link)
117 0.792 H006873_01 CGI-149 protein NM_016079 (external link) 189658 LOC51652 (external link)
118 0.592 H002066_01 Aldehyde dehydrogenase 1 family,
member A1
M31994 (external link) 76392 ALDH1A1 (external link)
119 0.689 H013490_01 H.sapiens polyA site DNA Z24724 (external link) 4934
120 0.76 H015305_01 Hypothetical protein FLJ22029 NM_024949 (external link) 285243 FLJ22029 (external link)
121 0.704 H002566_01 Membrane metallo-endopeptidase
(neutral endopeptidase, enkephalinase, CALLA, CD10)
NM_007289 (external link) 1298 MME (external link)
122 0.81 H002169_01 Sortilin-related receptor, L(DLR
class) A repeats-containing
NM_003105 (external link) 278571 SORL1 (external link)
123 0.88 H003649_01 Protein tyrosine phosphatase,
non-receptor type 6
X62055 (external link) 63489 PTPN6 (external link)
124 0.744 H011942_01 Activating transcription factor 5 AB021663 (external link) 9754 ATF5 (external link)
125 0.806 H007715_01 KIAA1091 protein AL117448 (external link) 26797 KIAA1091 (external link)
126 0.823 H012019_01 Epithelial protein lost in
neoplasm beta
NM_016357 (external link) 10706 EPLIN (external link)
127 0.838 H010079_01 Hypothetical protein FLJ10337 AB037858 (external link) 173484 KIAA1437 (external link)
128 0.772 H003031_01 Glycoprotein A repetitions
predominant
Z24680 (external link) 151641 GARP (external link)
129 0.762 H002785_01 Serine (or cysteine) proteinase
inhibitor, clade G (C1 inhibitor), member 1
X54486 (external link) 151242 SERPING1 (external link)
130 0.631 H000082_01 Deiodinase, iodothyronine, type II NM_013989 (external link) 154424 DIO2 (external link)
131 0.755 H007381_01 Hypothetical protein FLJ10044 AK000906 (external link) 127273 FLJ10044 (external link)
132 0.699 H014982_01 Tuftelin 1 NM_020127 (external link) 283009 TUFT1 (external link)
133 0.813 H003637_01 Protein tyrosine phosphatase,
non-receptor type 12
M93425 (external link) 62 PTPN12 (external link)
134 0.821 H002684_01 Bone morphogenetic protein 6 NM_001718 (external link) 285671 BMP6 (external link)
135 0.847 H001078_01 ATPase, Na+/K+ transporting, beta
3 polypeptide
NM_001679 (external link) 76941 ATP1B3 (external link)
136 0.785 H009413_01 KIAA0321 protein AB002319 (external link) 8663 KIAA0321 (external link)
137 0.806 H003439_01 Protein phosphatase 2 (formerly
2A), catalytic subunit, beta isoform
M60484 (external link) 80350 PPP2CB (external link)
138 0.811 H011488_01 UDP-Gal:beta GlcNAc beta 1,4-
galactosyltransferase, polypeptide 5
AL035683 (external link) 107526 B4GALT5 (external link)
139 0.631 H002334_01 Cytochrome P450, subfamily I
(dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile)
U03688 (external link) 154654 CYP1B1 (external link)
140 0.797 H006973_01 Homo sapiens cDNA FLJ10598 fis,
clone NT2RP2004841
AK001460 (external link) 170873
141 0.737 H008497_01 Amyloid beta (A4) precursor-like
protein 2
NM_016160 (external link) 279518 APLP2 (external link)
142 0.802 H000517_01 Phorbol-12-myristate-13-acetate-induced
protein 1
D90070 (external link) 96 PMAIP1 (external link)
143 0.799 H007506_01 Clock (mouse) homolog AB002332 (external link) 50722 CLOCK (external link)
144 0.831 H002305_01 Accessory proteins BAP31/BAP29 S74039 (external link) 291904 DXS1357E (external link)
145 0.813 H002571_01 Meiotic recombination (S.
cerevisiae) 11 homolog B
AF022778 (external link) 202379 MRE11B (external link)
146 0.795 H002782_01 Wee1+ (S. pombe) homolog X62048 (external link) 75188 WEE1 (external link)
147 0.814 H002076_01 Interleukin-1 receptor-associated
kinase 1
L76191 (external link) 182018 IRAK1 (external link)
148 0.82 H000048_01 PRKC, apoptosis, WT1, regulator U63809 (external link) 176090 PAWR (external link)
149 0.578 H005801_01 Purkinje cell protein 4 U52969 (external link) 80296 PCP4 (external link)
150 0.834 H002311_01 Transcription factor 7-like 2
(T-cell specific, HMG-box)
Y11306 (external link) 173638 TCF7L2 (external link)
151 0.774 H008367_01 KIAA0623 gene product AB014523 (external link) 151406 KIAA0623 (external link)
152 0.809 H005443_01 Interferon-related developmental
regulator 1
AC005192 (external link) 7879 IFRD1 (external link)
153 0.796 H005941_01 Hypothetical protein FLJ20043 AK000050 (external link) 103853 FLJ20043 (external link)
154 0.731 H009185_01 KIAA1144 protein AB032970 (external link) 22675 KIAA1144 (external link)
155 0.737 H011708_01 Homo sapiens clone 24405 mRNA
sequence
AF070632 (external link) 23729
156 0.848 H016365_01 Core1
UDP-galactose:N-acetylgalactosamine-alpha-R beta
1,3-galactosyltransferase
NM_020156 (external link) 46744 C1GALT1 (external link)
157 0.763 H010122_01 Homo sapiens mRNA; cDNA
DKFZp586F1223 (from clone DKFZp586F1223)
AL050204 (external link) 28540
158 0.71 H002475_01 Pim-1 oncogene M54915 (external link) 81170 PIM1 (external link)
159 0.794 H007334_01 SH3 domain binding glutamic
acid-rich protein like
AF042081 (external link) 14368 SH3BGRL (external link)
160 0.817 H000893_01 Major histocompatibility complex,
class I, E
X56841 (external link) 181392 HLA-E (external link)
161 0.737 H010951_01 LIM domain only 4 NM_006769 (external link) 3844 LMO4 (external link)
162 0.846 H001759_01 Chloride channel 7 AL031600 (external link) 80768 CLCN7 (external link)
163 0.845 H001612_01 RAP1, GTP-GDP dissociation
stimulator 1
AF237413 (external link) 7940 RAP1GDS1 (external link)
164 0.753 H007858_01 Glutaminyl-peptide
cyclotransferase (glutaminyl cyclase)
X71125 (external link) 79033 QPCT (external link)
165 0.808 H002897_01 Cathepsin L X12451 (external link) 78056 CTSL (external link)
166 0.84 H004099_01 Phosphatidic acid phosphatase
type 2A
AF014403 (external link) 41569 PPAP2A (external link)

SAM

‘Observed v. Expected’ table of GO classes and parent classes, in list of 166 genes shown above:

Only GO classes and parent classes with at least 5 observations in the selected subset and with an ‘Observed vs. Expected’ ratio of at least 2 are shown.

Cellular Component

GO id GO classification Observed in
selected subset
Expected in
selected subset
Observed/
Expected
0005887 integral to plasma membrane 19 9.37 2.03

Molecular Function

GO id GO classification Observed in
selected subset
Expected in
selected subset
Observed/
Expected
0016791 phosphoric monoester hydrolase activity 6 2.04 2.94
0005489 electron transporter activity 7 2.54 2.76
0042578 phosphoric ester hydrolase activity 6 2.4 2.5
0004713 protein-tyrosine kinase activity 5 2.16 2.31

Biological Process

GO id GO classification Observed in
selected subset
Expected in
selected subset
Observed/
Expected
0007600 sensory perception 6 1.92 3.13
0009581 detection of external stimulus 6 2.13 2.82
0006812 cation transport 6 2.93 2.05

SAM -ChromDist

View expression of significant genes.