Although congenital anomalies of the kidney and urinary tract (CAKUT) are the predominant birth defects diagnosed in the prenatal period and are the most common cause of pediatric end stage renal disease, only 13% of cases have a known monogenic cause. CAKUT can result in defects in the formation of nephrons, which are required for the function of the kidney. During kidney development, early beta-catenin-mediated Wnt signaling, which is required for nephric mesenchyme condensation into epithelia, transitions to planar cell polarity (PCP)-mediated Wnt signaling, resulting in morphogenesis and differentiation of the nephron. A balance between beta-catenin and PCP signaling is thus critical in kidney formation and maintenance, with disruption of this balance promoting cystic diseases and kidney cancers. Our laboratory uses Xenopus (frog) embryos to understand how cells communicate via Wnt signaling to coordinate nephron formation and how this process goes awry giving rise to CAKUT.
(Selected Publications and Preprints)
Kidney Int. 2022 Aug 30 [Epub ahead of print].
Corkins ME, DeLay BD, Miller RK.
Cold Spring Harb Protoc. 2021 Dec 15.
Krneta-Stankic V, Corkins ME, Paulucci-Holthauzen A, Kloc M, Gladden AB, Miller RK.
Cell Rep. 2021 Jul 6;36(1):109340.
Genesis. 2021 Feb;59(1-2):e23410.
Blackburn ATM, Bekheirnia N, Uma VC, Corkins ME, Xu Y, Rosenfeld JA, Bainbridge MN, Yang Y, Liu P, Madan-Khetarpal S, Delgado MR, Hudgins L, Krantz I, Rodriguez-Buritica D, Wheeler PG, Al Gazali L, Al Shamsi A, Gomez-Ospina N, Chao H, Mirzaa GM, Scheuerle AE, Kukolich MK, Scaglia F, Eng C, Willsey HR, Braun MC, Lamb DJ, Miller RK, Bekheirnia M.
Genet Med. 2019 Dec;21(12):2755-2764.
Divergent roles of the Wnt/PCP formin Daam1 in renal ciliogenesis
Corkins ME, Krneta-Stankic V, Kloc M, McCrea PD, Gladden AB, Miller RK.
PLoS One, 2019 Aug 30;14(8):e0221698.
Blackburn ATM, Miller RK.
Dis Model Mech. 2019 Apr 9;12(4).
DeLay BD, Baldwin TA, Miller RK.
Front. Physiol., 26 February 2019.
Corkins ME, Hanania HL, Krneta-Stankic V, DeLay BD, Pearl EJ, Lee M, Ji H, Davidson AJ, Horb ME, Miller RK.
Genes. 2018; 9(4).
DeLay BD, Corkins ME, Hanania HL, Salanga M, Deng JM, Sudou N, Taira M, Horb ME, Miller RK.
Genetics. 2018; 208(2):673-686.
Krneta-Stankic V, DeLay BD, Miller RK.
Pediatric nephrology (Berlin, Germany). 2017; 32(4):547-555.
Miller RK (Volume Editor)
Results and problems in cell differentiation. 2017, Springer Nature
Series Editors: Kubiak, Jacek Z., Kloc, Malgorzata.
DeLay BD, Krneta-Stankic V, Miller RK.
Journal of visualized experiments : JoVE. 2016; (111).
Miller RK, Lee M, McCrea PD.
1st ed. Kloc M, Kubiak JZ, editors. Oxford: John Wiley & Sons; 2014.
Chapter 12, The Xenopus Pronephros: A Kidney Model Making Leaps toward Understanding Tubule Development; p.215-238. 424p.
Miller RK, Hong JY, Muñoz WA, McCrea PD.
Progress in molecular biology and translational science. 2013; 116:387-407.
Miller RK, Canny SG, Jang CW, Cho K, Ji H, Wagner DS, Jones EA, Habas R, McCrea PD.
Journal of the American Society of Nephrology : JASN. 2011; 22(9):1654-64.
Miller RK, McCrea PD.
Developmental dynamics: an official publication of the American Association of Anatomists. 2010; 239(1):77-93.
Lyons JP, Miller RK, Zhou X, Weidinger G, Deroo T, Denayer T, Park JI, Ji H, Hong JY, Li A, Moon RT, Jones EA, Vleminckx K, Vize PD, McCrea PD.
Mechanisms of development. 2009; 126(3-4):142-59.