Antibody Engineering and Expression Core
Antibody therapeutics represents a major breakthrough in combating human diseases including cancer. Even though the pharmaceutical and biotechnology industries are in the center stage of drug discovery and development, academic researchers are increasingly engaged in discovering new antibody drug candidates. However, advancement of some the promising antibodies in the early stage of discovery from academic research laboratories is often hindered by the lack of access to the expertise and infrastructure required for antibody lead optimization. Our antibody engineering and expression service center will fill the gap of the much needed expertise for advancement of monoclonal antibodies from early discovery to lead optimization and preclinical development for the research and drug discovery communities. Objective of the service center is to provide technical support and services to advance proof-of-concept antibodies to the stage of preclinical development. Results generated from the core facility will strengthen the collaborators’ ability to attract external funding to continue development of the optimized therapeutic antibodies with the ultimate goal of translating basic research to novel therapies.
Contact Person for Antibody Engineering and Expression Service Center is:
Zhiqiang An, PhD, Center Director
Email: Zhiqiang.an@uth.tmc.edu
Phone: 713-500-3011
Ningyan Zhang, PhD, Co-Director
Email: Ningyan.Zhang@uth.tmc.edu
Phone: 713-500-3332
LOCATION:
IMM-TTI (Texas Therapeutics Institute)
SRB Room: 501R
Houston, TX77030
SERVICES:
The service center will contain four major functional modules: 1) Lead antibody generation; 2) Monoclonal antibody gene cloning and humanization; 3) Antibody affinity maturation; and 4) Cell line development & antibody production. List of services is shown below:
| SERVICE PROVIDED |
|---|
| MONOCLONAL ANTIBODY SERVICE |
| Antigen design and expression |
| Monoclonal antibody production from hybridoma cell line (mouse, rat and rabbit) |
| Molecular cloning of VH and VL from mouse, rat, rabbit hybridoma cell lines |
| Molecular cloning VH and VL from single B cells |
| ANTIBODY ENGINEERING |
| scFv production in E. coli |
| Full length IgG expression and purification in different isotypes (IgG1, IgG2, IgG4) in HEK293 or CHO cells |
| Humanization of monoclonal antibody from mouse, rat, rabbit or other animals |
| Antibody affinity maturation |
| Antibody affinity determination by surface plasmon resonance (SPR) method using Biacore T-100 |
| Polyclonal antibody purification |
| Epitope mapping |
