Transgenic Mouse & Stem Cells Core

The Transgenic and Stem Cells Service Center was established in 1998 and since that time, it has generated over 750 new transgenic, knock-out and knock-in mouse animal models for investigators from UTHealth, as well as for scientists from numerous other academic institutions. The stem cell lines that have been derived in the laboratory are highly effective for the generation of knock-out/ knock-in mice and for cell differentiation studies. These cell lines are routinely used in the Core Facility and are also commercially distributed. In addition to the production, cryopreservation and re-derivation of genetically-engineered mice and rats, the services of the facility also include gene targeting, using ES cells or CRISPR/Cas9 genome editing and intellectual/technical support in different aspects of microsurgery, cell culture and stem cells research.

Services:
• Microinjection of DNA, BAC or YAC clones for the production of transgenic mice
• Microinjection of ES cells for the production of knock-out and knock-in mice
• Microinjection of DNA for the production of transgenic rats
• Gene targeting, using ES cells or CRISPR/Cas9 genome editing
• Selection, expansion and cryopreservation of mouse ES cells
• Cryopreservation of fertilized mouse and rat eggs and sperm
• Re-derivation of mice and rats from fertilized eggs
• Availability of pathogen-free animal models from high level barrier facility
• Derivation of novel mouse ES cells and other cell lines
• Availability of germline competent mouse ES cell lines (129/SvImJ, C57BL/6 and BALB/c) and antibiotic -resistant mouse fibroblast feeder layer cells

Please use iLab to submit all service requests.

Contacts for further information: